66.2 Folate

66.2.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 250.7924480 1 <0.0001 ***
Study 3.2136720 1 0.07 ns
f39_gdm_new 0.5754347 1 0.4 ns
BMI 3.3368238 1 0.07 ns
Ethnicity_cauc 105.4219556 1 <0.0001 ***
f10c_any_cig_1st_vst 49.1590682 1 <0.0001 ***
GA_samp 164.6323095 1 <0.0001 ***
Study:f39_gdm_new 1.8652579 1 0.2 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 150.8759535 1 <0.0001 ***
Study 7.3986291 1 0.007 **
f39_gdm_new 0.4357432 1 0.5 ns
BMI 236.2094019 1 <0.0001 ***
Ethnicity_cauc 5.5165373 1 0.02 *
f10c_any_cig_1st_vst 0.9176455 1 0.3 ns
GA_samp 2601.9722143 1 <0.0001 ***
Study:f39_gdm_new 1.4845286 1 0.2 ns
GH2
Chisq Df Pr(>Chisq)
(Intercept) 251.096669 1 <0.0001 ***
Study 1.107622 1 0.3 ns
f39_gdm_new 1.386104 1 0.2 ns
BMI 87.622496 1 <0.0001 ***
Ethnicity_cauc 3.246832 1 0.07 ns
f10c_any_cig_1st_vst 1.039153 1 0.3 ns
GA_samp 1032.266869 1 <0.0001 ***
Study:f39_gdm_new 3.376951 1 0.07 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 21.326 21.326 <0.0001 ***
3 f39_gdm_new 1 Inf 33.726 33.726 <0.0001 ***
4 BMI 1 Inf 330.722 330.722 <0.0001 ***
5 Ethnicity_cauc 1 Inf 25.046 25.046 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 3.158 3.158 0.08 ns
7 GA_samp 1 Inf 2.506 2.506 0.1 ns
2 Study:f39_gdm_new 1 Inf 4.089 4.089 0.04 *
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 36.051 36.051 <0.0001 ***
3 f39_gdm_new 1 Inf 32.027 32.027 <0.0001 ***
4 BMI 1 Inf 316.807 316.807 <0.0001 ***
5 Ethnicity_cauc 1 Inf 24.882 24.882 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 2.618 2.618 0.1 ns
7 GA_samp 1 Inf 1.602 1.602 0.2 ns
2 Study:f39_gdm_new 1 Inf 4.361 4.361 0.04 *
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 53.182 53.182 <0.0001 ***
3 f39_gdm_new 1 Inf 4.345 4.345 0.04 *
4 BMI 1 Inf 11.449 11.449 0.0007 ***
5 Ethnicity_cauc 1 Inf 0.718 0.718 0.4 ns
6 f10c_any_cig_1st_vst 1 Inf 0.028 0.028 0.9 ns
7 GA_samp 1 Inf 0.278 0.278 0.6 ns
2 Study:f39_gdm_new 1 Inf 1.558 1.558 0.2 ns

66.2.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.75 (2.39, 3.12) <0.0001
Folate -0.00 (-0.00, 0.00) 0.12
f39_gdm_newGDM -0.11 (-0.35, 0.13) 0.36
StudySTOP -0.08 (-0.21, 0.05) 0.23
GA_samp 0.14 (0.12, 0.16) <0.0001
Folate:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.75
f39_gdm_newGDM:StudySTOP 0.16 (-0.07, 0.38) 0.17
Folate:StudySTOP 0.00 (-0.00, 0.00) 0.10
HPL
Var Effect P
(Intercept) 0.65 (0.46, 0.84) <0.0001
Folate -0.00 (-0.00, 0.00) 0.58
f39_gdm_newGDM -0.11 (-0.23, 0.01) 0.07
StudySTOP 0.10 (0.03, 0.17) 0.004
GA_samp 0.29 (0.28, 0.30) <0.0001
Folate:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.28
f39_gdm_newGDM:StudySTOP 0.06 (-0.05, 0.17) 0.31
Folate:StudySTOP -0.00 (-0.00, 0.00) 0.89
GH2
Var Effect P
(Intercept) -2.57 (-2.82, -2.32) <0.0001
Folate -0.00 (-0.00, 0.00) 0.37
f39_gdm_newGDM -0.17 (-0.34, 0.00) 0.05
StudySTOP -0.01 (-0.11, 0.08) 0.77
GA_samp 0.26 (0.25, 0.28) <0.0001
Folate:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.65
f39_gdm_newGDM:StudySTOP 0.17 (0.00, 0.33) 0.05
Folate:StudySTOP -0.00 (-0.00, 0.00) 0.89
Insulin
Var Effect P
(Intercept) 2.45 (1.99, 2.91) <0.0001
Folate -0.00 (-0.00, 0.00) 0.56
f39_gdm_newGDM 0.94 (0.44, 1.44) 0.0002
StudySTOP -0.17 (-0.36, 0.02) 0.07
GA_samp 0.02 (-0.01, 0.05) 0.24
Folate:f39_gdm_newGDM -0.00 (-0.01, 0.00) 0.27
f39_gdm_newGDM:StudySTOP -0.42 (-0.98, 0.13) 0.14
Folate:StudySTOP -0.00 (-0.00, 0.00) 0.24
Folate:f39_gdm_newGDM:StudySTOP 0.00 (-0.00, 0.01) 0.38
HOMA.IR
Var Effect P
(Intercept) 1.09 (0.60, 1.58) <0.0001
Folate -0.00 (-0.00, 0.00) 0.54
f39_gdm_newGDM 1.08 (0.52, 1.65) 0.0002
StudySTOP -0.32 (-0.51, -0.12) 0.002
GA_samp 0.01 (-0.02, 0.04) 0.39
Folate:f39_gdm_newGDM -0.00 (-0.01, 0.00) 0.18
f39_gdm_newGDM:StudySTOP -0.51 (-1.13, 0.10) 0.10
Folate:StudySTOP -0.00 (-0.00, 0.00) 0.25
Folate:f39_gdm_newGDM:StudySTOP 0.00 (-0.00, 0.01) 0.33
GLUC3_mmol.L
Var Effect P
(Intercept) 1.73 (1.63, 1.82) <0.0001
Folate -0.00 (-0.00, 0.00) 0.67
f39_gdm_newGDM 0.16 (0.03, 0.30) 0.02
StudySTOP -0.15 (-0.18, -0.11) <0.0001
GA_samp -0.00 (-0.01, 0.00) 0.44
Folate:f39_gdm_newGDM -0.00 (-0.00, -0.00) 0.03
f39_gdm_newGDM:StudySTOP -0.07 (-0.19, 0.04) 0.20
Folate:StudySTOP 0.00 (-0.00, 0.00) 0.61

66.2.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Serum folate concentration (nM) and may be misleading when interaction term is involved.

Prolactin
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 95.27842 7.245025 92.02225 81.92307 110.8110
STOP non-GDM 95.99562 6.888971 73.23520 83.20322 110.7548
SCOPE GDM 87.47004 11.007528 554.68775 68.31356 111.9984
STOP GDM 103.24476 9.012600 156.11767 86.89267 122.6741
HPL
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 89.05209 2.458770 174.9519 84.32927 94.03941
STOP non-GDM 98.36258 2.429469 113.9478 93.66564 103.29505
SCOPE GDM 83.68200 4.640853 830.6800 75.05110 93.30545
STOP GDM 98.04443 3.614067 443.9313 91.19280 105.41084
GH2
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 2.333066 0.0707105 454.5683 2.198164 2.476248
STOP non-GDM 2.286500 0.0564626 253.5503 2.177965 2.400443
SCOPE GDM 2.021693 0.1569705 1368.5699 1.736068 2.354310
STOP GDM 2.336972 0.1025856 821.6759 2.144042 2.547262
Insulin
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 14.01040 0.7336892 Inf 12.64374 15.52480
STOP non-GDM 10.80410 0.4091616 Inf 10.03120 11.63655
SCOPE GDM 30.04116 4.0282239 Inf 23.09824 39.07099
STOP GDM 17.98223 1.3989404 Inf 15.43916 20.94418
HOMA.IR
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 3.389311 0.1887886 Inf 3.038775 3.780283
STOP non-GDM 2.244756 0.0897830 Inf 2.075505 2.427809
SCOPE GDM 7.879545 1.1696777 Inf 5.890398 10.540413
STOP GDM 3.841241 0.3191235 Inf 3.264038 4.520515
GLUC3_mmol.L
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 5.421737 0.0532507 Inf 5.318366 5.527117
STOP non-GDM 4.708414 0.0380618 Inf 4.634402 4.783608
SCOPE GDM 5.964490 0.3009833 Inf 5.402808 6.584566
STOP GDM 4.810264 0.0606364 Inf 4.692875 4.930589

66.2.4 Comparisons

66.2.4.1 Within Groups

Effect for every 50 unit increase in Serum folate concentration (nM).
Hormone Contrast Study f39_gdm_new Ratio of Geometric means (95% CI) P-value
Prolactin Folate89.2 / Folate39.2 SCOPE non-GDM 0.949 (0.888, 1.013) 0.1
Prolactin Folate89.2 / Folate39.2 STOP non-GDM 1.017 (0.964, 1.072) 0.5
Prolactin Folate89.2 / Folate39.2 SCOPE GDM 0.968 (0.846, 1.106) 0.6
Prolactin Folate89.2 / Folate39.2 STOP GDM 1.037 (0.927, 1.160) 0.5
HPL Folate89.2 / Folate39.2 SCOPE non-GDM 0.990 (0.956, 1.025) 0.6
HPL Folate89.2 / Folate39.2 STOP non-GDM 0.987 (0.959, 1.016) 0.4
HPL Folate89.2 / Folate39.2 SCOPE GDM 1.027 (0.955, 1.104) 0.5
HPL Folate89.2 / Folate39.2 STOP GDM 1.024 (0.962, 1.089) 0.5
GH2 Folate89.2 / Folate39.2 SCOPE non-GDM 0.978 (0.932, 1.027) 0.4
GH2 Folate89.2 / Folate39.2 STOP non-GDM 0.974 (0.936, 1.013) 0.2
GH2 Folate89.2 / Folate39.2 SCOPE GDM 0.999 (0.904, 1.104) 1.0
GH2 Folate89.2 / Folate39.2 STOP GDM 0.994 (0.913, 1.083) 0.9
Insulin Folate89.2 / Folate39.2 SCOPE non-GDM 0.971 (0.880, 1.072) 0.6
Insulin Folate89.2 / Folate39.2 STOP non-GDM 0.906 (0.850, 0.965) 0.002
Insulin Folate89.2 / Folate39.2 SCOPE GDM 0.848 (0.681, 1.056) 0.1
Insulin Folate89.2 / Folate39.2 STOP GDM 0.903 (0.767, 1.063) 0.2
HOMA.IR Folate89.2 / Folate39.2 SCOPE non-GDM 0.968 (0.870, 1.076) 0.5
HOMA.IR Folate89.2 / Folate39.2 STOP non-GDM 0.898 (0.840, 0.961) 0.002
HOMA.IR Folate89.2 / Folate39.2 SCOPE GDM 0.802 (0.624, 1.031) 0.09
HOMA.IR Folate89.2 / Folate39.2 STOP GDM 0.875 (0.739, 1.036) 0.1
GLUC3_mmol.L Folate89.2 / Folate39.2 SCOPE non-GDM 0.997 (0.981, 1.013) 0.7
GLUC3_mmol.L Folate89.2 / Folate39.2 STOP non-GDM 1.003 (0.986, 1.020) 0.7
GLUC3_mmol.L Folate89.2 / Folate39.2 SCOPE GDM 0.946 (0.895, 1.000) 0.05
GLUC3_mmol.L Folate89.2 / Folate39.2 STOP GDM 0.952 (0.915, 0.990) 0.01

66.2.4.2 Between Groups

Below are comparisons of the differences in the change in Serum folate concentration (nM) between groups. I.e. whether the change in Serum folate concentration (nM) are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.148847 0.0976187 2094.601 0.9725075 1.357161 0.103 ns
STOP/SCOPE (non-GDM) 1.071843 0.0455378 2094.601 0.9861579 1.164973 0.103 ns
STOP/SCOPE (GDM) 1.071843 0.0455378 2094.601 0.9861579 1.164973 0.103 ns
GDM/non-GDM (SCOPE) 1.019961 0.0632601 2037.473 0.9031484 1.151883 0.750 ns
GDM/non-GDM (STOP) 1.019961 0.0632601 2037.473 0.9031484 1.151883 0.750 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9937029 0.0451440 1994.048 0.9089979 1.086301 0.889 ns
STOP/SCOPE (non-GDM) 0.9968465 0.0226434 1994.048 0.9534138 1.042258 0.889 ns
STOP/SCOPE (GDM) 0.9968465 0.0226434 1994.048 0.9534138 1.042258 0.889 ns
GDM/non-GDM (SCOPE) 1.0373673 0.0352087 1984.351 0.9705653 1.108767 0.280 ns
GDM/non-GDM (STOP) 1.0373673 0.0352087 1984.351 0.9705653 1.108767 0.280 ns
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9911261 0.0626622 2132.661 0.8755533 1.121954 0.888 ns
STOP/SCOPE (non-GDM) 0.9955531 0.0314711 2132.661 0.9357101 1.059224 0.888 ns
STOP/SCOPE (GDM) 0.9955531 0.0314711 2132.661 0.9357101 1.059224 0.888 ns
GDM/non-GDM (SCOPE) 1.0212705 0.0478383 2124.039 0.9316357 1.119529 0.653 ns
GDM/non-GDM (STOP) 1.0212705 0.0478383 2124.039 0.9316357 1.119529 0.653 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 0.9934602 0.1505893 Inf 0.7381163 1.337138 0.965 ns
STOP/SCOPE (non-GDM) 0.9324543 0.0559244 Inf 0.8290416 1.048767 0.244 ns
STOP/SCOPE (GDM) 1.0654250 0.1483656 Inf 0.8109404 1.399771 0.649 ns
GDM/non-GDM (SCOPE) 0.8728867 0.1071022 Inf 0.6863039 1.110195 0.268 ns
GDM/non-GDM (STOP) 0.9973629 0.0889477 Inf 0.8374147 1.187862 0.976 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0131275 0.1695444 Inf 0.7298230 1.406406 0.938 ns
STOP/SCOPE (non-GDM) 0.9282432 0.0596406 Inf 0.8184105 1.052816 0.246 ns
STOP/SCOPE (GDM) 1.0914462 0.1686783 Inf 0.8062185 1.477583 0.571 ns
GDM/non-GDM (SCOPE) 0.8288632 0.1155367 Inf 0.6307138 1.089265 0.178 ns
GDM/non-GDM (STOP) 0.9745933 0.0903565 Inf 0.8126561 1.168799 0.781 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0130189 0.0258700 Inf 0.9635626 1.0650136 0.613 ns
STOP/SCOPE (non-GDM) 1.0064884 0.0128516 Inf 0.9816123 1.0319950 0.613 ns
STOP/SCOPE (GDM) 1.0064884 0.0128516 Inf 0.9816123 1.0319950 0.613 ns
GDM/non-GDM (SCOPE) 0.9490467 0.0230496 Inf 0.9049288 0.9953155 0.031 *
GDM/non-GDM (STOP) 0.9490467 0.0230496 Inf 0.9049288 0.9953155 0.031 *

66.2.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

66.2.6 Plots